Mobile Phase Considerations in LPLC
Mobile phase optimization is essential for achieving efficient separation in LPLC. Buffers must maintain pH stability and ensure compatibility with proteins or other biomolecules. Salt gradients, organic modifiers, and additives can be adjusted to enhance selectivity.
Researchers must also consider factors such as ionic strength, viscosity, and temperature. Proper filtration and degassing of solvents prevent inconsistencies during purification. By fine-tuning the mobile phase, scientists can significantly improve resolution and purification efficiency.
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